Difference between revisions of "Part:BBa K3018000"
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<partinfo>BBa_K3018000 short</partinfo> | <partinfo>BBa_K3018000 short</partinfo> | ||
| − | This part encodes a bacterial flavin-containing monooxygenase( | + | This part encodes a bacterial flavin-containing monooxygenase(bFMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli. |
Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works. | Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works. | ||
Revision as of 07:25, 14 October 2019
Bacterial flavin-containing monooxydase, codon-optimized
This part encodes a bacterial flavin-containing monooxygenase(bFMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli. Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]