Difference between revisions of "Part:BBa K3140002"

Revision as of 06:48, 14 October 2019

PsiK - 4-hydroxytryptamine kinase from Psilocybe cubensis

PsiK is a 4-hydroxytryptamine kinase, which catalyses the conversion of 4-hydroxytryptamine to norbaeocystin.

NCBI: ASU62237.1
UniProt: P0DPA8
EC number: 2.7.1.222

Usage and Biology

PsiK is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: Phosphorylation of 4-hydroxytryptamine to norbaeocystin, mediated by PsiK. The reaction is dependant upon ATP, and ADP is released as a by-product. Source: KEGG

Fig. 2: Phosphorylation of psilocin to psilocybin, mediated by PsiK. The reaction is dependant upon ATP, and ADP is released as a by-product. Source: KEGG

ADD: -SDS-PAGE - pCW - LC/MAS

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 864
Illegal SapI.rc site found at 973

@@ Line 13: / Line 13: @@
 PsiK is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
-[[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685 KEGG]  ]]
+[[Image:T--Sydney_Australia--PsiK_KEGG_rxn1.gif|thumb|900px|Fig. 1: Phosphorylation of 4-hydroxytryptamine to norbaeocystin, mediated by PsiK. The reaction is dependant upon ATP, and ADP is released as a by-product. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R11933 KEGG]  ]]
+[[Image:T--Sydney_Australia--PsiK_KEGG_rxn2.gif|thumb|900px|Fig. 2: Phosphorylation of psilocin to psilocybin, mediated by PsiK. The reaction is dependant upon ATP, and ADP is released as a by-product. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R08480 KEGG]  ]]