Difference between revisions of "Part:BBa K3140001"
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PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine. | PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine. | ||
| − | *[https://www.ncbi.nlm.nih.gov/protein/ASU62246.1 | + | *NCBI: [https://www.ncbi.nlm.nih.gov/protein/ASU62246.1 ASU62246.1] |
*UniProt: [https://www.uniprot.org/uniprot/P0DPA7 P0DPA7] | *UniProt: [https://www.uniprot.org/uniprot/P0DPA7 P0DPA7] | ||
*EC number: [https://enzyme.expasy.org/EC/1.14.99.59 1.14.99.59] | *EC number: [https://enzyme.expasy.org/EC/1.14.99.59 1.14.99.59] | ||
Revision as of 06:49, 14 October 2019
PsiH - Cytochrome P450 monooxygenase from Psilocybe cubensis
PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.
- NCBI: ASU62246.1
- UniProt: P0DPA7
- EC number: 1.14.99.59
Usage and Biology
PsiH is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in Escherichia coli <add strain> in a <expression system>.
Fig. 1: Hydroxylation of tryptamine to 4-hydroxytryptamine, mediated by PsiH. The reaction relies on a hydrogen donor (C00030) and O2, which results in the release of the oxidised donor (C00028) and H2O as a by-product. Source: KEGG
ADD: -SDS-PAGE - pCW - LC/MAS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]