Difference between revisions of "Part:BBa K3110002:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
lldD has an EcoRI site, which we couldn't eliminate and hence the part needs further improvement.
+
The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This has been amplified from the genome.
+
lldD got synthesized by Twist Biosciences and lldP was obtained from the genomic DNA of <i>Ecoli</I>  Dh5&alpha;. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).
  
 
===References===
 
===References===

Revision as of 12:58, 14 October 2019


lldP+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2805
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2242


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

lldD got synthesized by Twist Biosciences and lldP was obtained from the genomic DNA of Ecoli Dh5α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).

References