Difference between revisions of "Part:BBa K3140000"
| Line 13: | Line 13: | ||
PsiD is a native enzyme obtained from the ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | PsiD is a native enzyme obtained from the ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | ||
| − | [[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. Source: KEGG]] | + | [[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685+R11932 KEGG] ]] |
ADD: | ADD: | ||
Revision as of 05:18, 14 October 2019
PsiD - Tryptophan decarboxylase from Psilocybe cubensis
PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.
Usage and Biology
PsiD is a native enzyme obtained from the Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in Escherichia coli <add strain> in a <expression system>.
Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. Source: KEGG
ADD:
- SDS-PAGE IMAGE - LC/MS - pUS250 and pET-28c(+)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]