Difference between revisions of "Part:BBa K3253003"

Revision as of 00:58, 15 October 2019

Ghchi 6

This gene is found in red chicken-feet cotton, and it belongs to Glyco-hydro-19's subfamily. This gene is composed of 975 bases and codes for the synthesis of class I chitinase which is made up of 324 amino acids.Its molecular weight is 34.65 k Da and isoelectric point is 7.81. Through amino acid sequence comparison, cotton gene Ghchi6 and other plant gene are highly conservative, and is evolutionarily close relative to chitin of woody cotton. When the cotton is physically attacked by aphids, the gene Ghchi6 gets overexpressed that the plant starts to secrete chitinase. The overexpression of Ghchi6 can improve the aphid-resistance in cotton, and we plan to transfer this favored gene to tobacco to improve its aphid-resistance. Through modifications we have transferred the gene to Agrobacterium and then to a common tobacco Nicotiana rustica through infection. Normally the expression of chitinase in plants is very low under common conditions, but in cottons the expression is triggered by external factors like mechanical damage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3253003 short</partinfo>
-This gene is found in red chicken-feet cotton, and it belongs to Glyco-hydro-19's subfamily. This gene is composed of 975 bases and codes for the synthesis of class I chitinase which is made up of 324 amino acids. When the cotton is physically attacked by aphids, the gene Ghchi6 gets overexpressed that the plant starts to secrete chitinase. The overexpression of Ghchi6 can improve the aphid-resistance in cotton, and we plan to transfer this favored gene to tobacco to improve its aphid-resistance. Through modifications we have transferred the gene to Agrobacterium and then to the tobacco through infection. Normally the expression of chitinase in plants is very low under common conditions, but in cottons the expression is triggered by external factors like mechanical damage.
+This gene is found in red chicken-feet cotton, and it belongs to Glyco-hydro-19's subfamily. This gene is composed of 975 bases and codes for the synthesis of class I chitinase which is made up of 324 amino acids.Its  molecular  weight  is  34.65  k Da  and  isoelectric  point  is  7.81. Through amino acid sequence comparison, cotton gene Ghchi6 and other plant gene are  highly  conservative,  and  is  evolutionarily  close  relative  to  chitin  of  woody cotton.
+When the cotton is physically attacked by aphids, the gene Ghchi6 gets overexpressed that the plant starts to secrete chitinase. The overexpression of Ghchi6 can improve the aphid-resistance in cotton, and we plan to transfer this favored gene to tobacco to improve its aphid-resistance. Through modifications we have transferred the gene to Agrobacterium and then to a common tobacco Nicotiana rustica through infection. Normally the expression of chitinase in plants is very low under common conditions, but in cottons the expression is triggered by external factors like mechanical damage.
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