Difference between revisions of "Part:BBa K3140001"

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PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.
 
PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.
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*[https://www.ncbi.nlm.nih.gov/protein/ASU62246.1 NCBI]
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*UniProt: [https://www.uniprot.org/uniprot/P0DPA7 P0DPA7]
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*EC number: [https://enzyme.expasy.org/EC/1.14.99.59 1.14.99.59]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 06:30, 14 October 2019


PsiH - Cytochrome P450 monooxygenase from Psilocybe cubensis

PsiH is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.

Usage and Biology

PsiH is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in Escherichia coli <add strain> in a <expression system>.

ADD: -SDS-PAGE - pCW - LC/MAS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]