Difference between revisions of "Part:BBa K3140000"
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PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.
 
PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:02, 14 October 2019


PsiD - Tryptophan decarboxylase from Psilocybe cubensis

PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.

Usage and Biology

PsiD is a native enzyme obtained from the Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in Escherichia coli <add strain> in a <expression system>.

ADD:

- SDS-PAGE IMAGE - LC/MS - pUS250 and pET-28c(+)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]