Difference between revisions of "Part:BBa K2912007"

 
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<partinfo>BBa_K2912007 short</partinfo>
 
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This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein LHCB7 of M. micrantha, through which we can block this essential matabolic gene expression to kill such invasive weed.
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This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein LHCB7 of M. micrantha（Unigene0025307）, through which we can block this essential matabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.
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<center><html><img src='https://2019.igem.org/wiki/images/f/fb/T--SZU-CHINA--fig._1%EF%BC%883%EF%BC%89.png' style="width:50%;margin:0 auto">
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<center> Fig.1 The Relative Target Gene Expression（Unigene0025307） after Using hairpin siRNA </center></html></center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:33, 19 October 2019


M. micrantha_leaves_ Unigene0025307

This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein LHCB7 of M. micrantha（Unigene0025307）, through which we can block this essential matabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.

Fig.1 The Relative Target Gene Expression（Unigene0025307） after Using hairpin siRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]