Difference between revisions of "Part:BBa K3142013"
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<partinfo>BBa_K3142013 parameters</partinfo> | <partinfo>BBa_K3142013 parameters</partinfo> | ||
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| + | ==SZPT-China== | ||
| + | To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited. | ||
| + | [[file:p-a.png|center|500px]] | ||
Revision as of 19:24, 19 October 2019
acmA
what it is：
what it does：
how to use it in their projects：
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 627
Illegal PstI site found at 151 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 151
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 579
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 627
Illegal PstI site found at 151 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 627
Illegal PstI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
SZPT-China
To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.
