Difference between revisions of "Part:BBa K3019012:Design"
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===Source=== | ===Source=== | ||
Latest revision as of 18:02, 13 October 2019
α-factor pre-pro signal BBa_K2711000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 402
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 517
Illegal BamHI site found at 737 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 406
Illegal NgoMIV site found at 768 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Illegal cutting sites removal
Source
BBa_K2711000 sequence is taken from iGEM registry, Alfa factor pre-pro signal sequence was taken from yeast genomic DNA.