Difference between revisions of "Part:BBa K3117020:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | This part was codon optimized for expression in mammalian cells. | ||
===Source=== | ===Source=== | ||
| − | . | + | The sequence of this part was provided by Prof. Matthias Peipp from the Christian-Albrechts-Universität of Kiel. |
===References=== | ===References=== | ||
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| + | |||
| + | [1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032. | ||
Revision as of 22:30, 21 October 2019
scFv VH against CD3 codon optimized for CHO expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 142
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was codon optimized for expression in mammalian cells.
Source
The sequence of this part was provided by Prof. Matthias Peipp from the Christian-Albrechts-Universität of Kiel.
References
[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.