Difference between revisions of "Part:BBa K143031"

m
m
Line 3: Line 3:
  
 
Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''<cite>#1</cite>. Expression in a host confers resistance to spectinomycin at a concentration of 100&#956;g/&#956;l and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870<cite>#2</cite>, pCOMT-Kan<cite>#3</cite> and pIEF16s<cite>#4</cite>
 
Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''<cite>#1</cite>. Expression in a host confers resistance to spectinomycin at a concentration of 100&#956;g/&#956;l and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870<cite>#2</cite>, pCOMT-Kan<cite>#3</cite> and pIEF16s<cite>#4</cite>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 16: Line 20:
 
<partinfo>BBa_K143031 parameters</partinfo>
 
<partinfo>BBa_K143031 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===References===
 +
 +
<biblio>
 +
#1 pmid=1659306
 +
#2 pmid=16997985
 +
#3 pmid=15060042
 +
#4 pmid=16714443
 +
</biblio>

Revision as of 11:38, 18 September 2008

Aad9 Spectinomycin Resistance Gene

Aad9 is the spectinomycin resistance gene from Enterococcus faecalis#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both B. subtilis and E. coli including pDP870#2, pCOMT-Kan#3 and pIEF16s#4



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488


References

<biblio>

  1. 1 pmid=1659306
  2. 2 pmid=16997985
  3. 3 pmid=15060042
  4. 4 pmid=16714443

</biblio>