Difference between revisions of "Part:BBa K2922038:Design"

 
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===Design Notes===
 
===Design Notes===
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Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010)
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Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) in the end of operon`s sequence(BBa_B0010)
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+
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===Source===
 
===Source===

Revision as of 16:27, 13 October 2019


The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1938
    Illegal NheI site found at 1961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
    Illegal AgeI site found at 1602
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) in the end of operon`s sequence(BBa_B0010)

Source

This part constructs from BBa_K525998,BBa_K2922027,BBa_K2922028,BBa_K2922029,BBa_K1033917 by standard assembly.

References