Difference between revisions of "Part:BBa K3278001"

Revision as of 11:18, 16 October 2019

pDawn with T7 promoter

pDawn is a blue light control system which can start the expression of target gene under the inducement of blue light. More information in BBa_K1075044 added by iGEM13_Bonn.

However, expression leakage and delay are the two main problems of pdawn system. Leakage refers to the expression of target protein without light. According to our measurement, samples strictly kept away from light still had significant expression of GFP after being cultured for one night. Therefore, we carefully studied the gene circuits of pdawn system [2] [3] and hypothesized that enhancing the strength of the promoter of YF1 may enhance the repression of protein expression in the dark, thus solving the leakage problem.

In this part, we use the T7 promoter to substitute the lacIq promoter in front of YF1 and Fixk2, largely reducing the leakage while maintain reasonable expression under blue light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2231
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 109
Illegal NgoMIV site found at 241
Illegal NgoMIV site found at 335
Illegal NgoMIV site found at 628
Illegal NgoMIV site found at 1122
Illegal NgoMIV site found at 1140
Illegal NgoMIV site found at 1230
Illegal AgeI site found at 460
Illegal AgeI site found at 1588
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1683
Illegal BsaI.rc site found at 571

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3278001 short</partinfo>
-Change one of the promoters of pDawn system lacIq to T7 promoter.
+pDawn is a blue light control system which can start the expression of target gene under the inducement of blue light. More information in BBa_K1075044 added by iGEM13_Bonn.
+However, expression leakage and delay are the two main problems of pdawn system. Leakage refers to the expression of target protein without light. According to our measurement, samples strictly kept away from light still had significant expression of GFP after being cultured for one night. Therefore, we carefully studied the gene circuits of pdawn system [2] [3] and hypothesized that enhancing the strength of the promoter of YF1 may enhance the repression of protein expression in the dark, thus solving the leakage problem.
+In this part, we use the T7 promoter to substitute the lacIq promoter in front of YF1 and Fixk2, largely reducing the leakage while maintain reasonable expression under blue light.
 <!-- Add more about the biology of this part here