Difference between revisions of "Part:BBa K2923021:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part was created by Gibson assembly. | This part was created by Gibson assembly. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 14:52, 13 October 2019
_NOTOC__ MS2-MS2-Guanine inactif aptazyme-PP7-PP7
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 25
Illegal PstI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by Gibson assembly.
Source
This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. The guanine aptazyme sequence is previously described by Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.
References
Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46 Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.