Difference between revisions of "Part:BBa K2963032"
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<partinfo>BBa_K2963032 short</partinfo>
 
<partinfo>BBa_K2963032 short</partinfo>
  
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This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme by using the "Ptac-lacO-rbs-racE-T7t" part in order to make comparison with "BBa_K2963033" which contains two lacO.
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This part contains the racE racemase gene, which was constructed using the tac (BBa_K864400) promoter, rbs (BBa_K2963006), racE gene (BBa_K2963004) and the T7 terminator (BBa_K2598024). This part was compared with the other part (BBa_K2963033) which contained another lac operator follwowing the tac promoter（BBa_K864400）.Simultaneously, the rest basic parts are the same. The racemase activity data and real-time PCR data were used to characterize the composite part. And we compared the different expression levels of racE gene of the two composite (BBa_K2963033 and BBa_K2963032）.
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===Usage and Biology===
 
===Usage and Biology===
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The racE gene is a type of racemase gene from Bacillus subtilis (the glutamate racemase gene in Bacillus licheniformis is glr). According to reports, racE(glr) has weak affinity and strong catalytic properties against L-glutamic acid. And the racemase gene appears to be an essential gene for the catabolism of exogenous D-glutamic acid produced by γ-PGA.
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The racE gene is a type of racemase gene from Bacillus subtilis while in Bacillus licheniformis is named glr. According to previous reports, glr has two characters against L-glutamic acid, one is weak affinity and the other is strong catalytic properties.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:51, 13 October 2019


racE- encoding racemase

This part contains the racE racemase gene, which was constructed using the tac (BBa_K864400) promoter, rbs (BBa_K2963006), racE gene (BBa_K2963004) and the T7 terminator (BBa_K2598024). This part was compared with the other part (BBa_K2963033) which contained another lac operator follwowing the tac promoter（BBa_K864400）.Simultaneously, the rest basic parts are the same. The racemase activity data and real-time PCR data were used to characterize the composite part. And we compared the different expression levels of racE gene of the two composite (BBa_K2963033 and BBa_K2963032）. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]