Difference between revisions of "Part:BBa K2923008"
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| − | An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003 | + | An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923003</partinfo> |
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| + | [[Gua active et inactive.jpg|300px|thumb|center|Figure: Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.] | ||
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Revision as of 08:09, 14 October 2019
Guanine inactif aptazyme
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003
![300px|thumb|center|Figure: Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]](/wiki/index.php?title=Gua_active_et_inactive.jpg&action=edit&redlink=1){kind=link}
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]