Difference between revisions of "Part:BBa K2984005"
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The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA).
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<partinfo>BBa_K2984006 short</partinfo>
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA).
 
In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
 
In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2984006 SequenceAndFeatures</partinfo>

Revision as of 13:51, 17 October 2019

Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii

This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 298
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]