Difference between revisions of "Part:BBa K2923002:Design"
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===Design Notes===
 
===Design Notes===
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Aptazyme is composed of three different part: theo5 as a communication module, P1 as a twister ribozyme mutated in the 14 and 15 positions (GT→TG) to inhibit the RNA cleavage activity of the ribozyme part and aptamer module specific to theophylline.
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Aptazyme is composed of three different parts: Theo5 as a communication module, aptamer module specific to theophylline and P1 as a twister ribozyme mutated in the 14 and 15 positions (GT→TG) to inhibit the RNA cleavage activity. The no highlighted sequences correspond to the riboswitch module.
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The no highlighted sequences correspond to the riboswitch module.
+
 
  
  

Revision as of 10:57, 13 October 2019

Theophylline inactif aptazyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aptazyme is composed of three different parts: Theo5 as a communication module, aptamer module specific to theophylline and P1 as a twister ribozyme mutated in the 14 and 15 positions (GT→TG) to inhibit the RNA cleavage activity. The no highlighted sequences correspond to the riboswitch module.



Source

Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7