Difference between revisions of "Part:BBa K3002017:Experience"
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| − | + | <p>We detected the expression of the POR with HA-tag via immunoblotting.</p> | |
| + | <p> | ||
| + | <img class="bild" src="https://static.igem.wiki/teams/4806/wiki/registry/level2/por-ha-wb.png"> | ||
| + | <div class="unterschrift"><b>Fig.2 Expression of the POR with HA-tag</b><br> | ||
| + | (a)Level 2 MoClo construct for expression of the POR containing the HA-tag was designed (see Fig.1 for part description) <br> (b) Picture of resulting western blot. The POR is marked by a black arrow, the white arrow marks a cross reaction of antibodies. For reference, the UVM4 recipient strain and a strain expressing the HA-tagged ribosomal chloroplast 50S protein L5 (RPL5) were used as a negative and positive control, respectively | ||
| + | </div> | ||
| + | </p> | ||
| + | <p>For detection the UVM4 strain was transformed with the construct in (a). 30 hygromycin-resistant transformants were cultivated in TAP medium and samples were taken after 3 days. Whole-cell proteins were extracted and analyzed by SDS-PAGE and immunoblotting using an anti-HA antibody. The expression of the POR (~ 77 kDa) is visible. | ||
| + | </p> | ||
|}; | |}; | ||
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Revision as of 23:57, 11 October 2023
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