Difference between revisions of "Part:BBa K2923002:Design"
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The anti-theophylline aptazyme is described by Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7
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__NOTOC__
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<partinfo>BBa_K2923007 short</partinfo>
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<partinfo>BBa_K2923007 SequenceAndFeatures</partinfo>
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===Design Notes===
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=Aptazyme is composed of three different part :
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=Theo5 as a communication module,
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=P1 as a twister ribozyme mutated in the 14 an 15 position to inhibit the RNA cleavage activity of the ribozyme part
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=Aptamer module specific to theophylline.
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===Source===
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Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7

Revision as of 09:34, 13 October 2019

aptazyme theophylline inactif


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

=Aptazyme is composed of three different part : =Theo5 as a communication module, =P1 as a twister ribozyme mutated in the 14 an 15 position to inhibit the RNA cleavage activity of the ribozyme part =Aptamer module specific to theophylline.



Source

Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7