Difference between revisions of "Part:BBa K3179004:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Adenovirus protein
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This part is constructed by adding 2X Kturn sequence before Adenovirus E1A gene. Due to the existence of 2X Kturn, the translation of E1A is controlled by L7Ae. E1A is an early gene of adenovirus and is essential in the commence of viral replication.
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+
This part has an intron which also exists in E1A in the original adenoviral genome. Thus using the splicing system in eukaryotes can make it function as normal. In our design, 2kt-E1A is used as a response part to startup viral gene expression and lyse target cells.
  
 +
We integrate 2kt-E1 with miR885-5p-target and miR663b-target so that while miRNA885-5p and miR663b-target are absent in the cell, E1A can be translated to startup viral gene expression and lyse target cells.
  
 
===Source===
 
===Source===

Latest revision as of 19:28, 19 October 2019


2Kt-E1A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 859
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 859
    Illegal NgoMIV site found at 333
    Illegal AgeI site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed by adding 2X Kturn sequence before Adenovirus E1A gene. Due to the existence of 2X Kturn, the translation of E1A is controlled by L7Ae. E1A is an early gene of adenovirus and is essential in the commence of viral replication. This part has an intron which also exists in E1A in the original adenoviral genome. Thus using the splicing system in eukaryotes can make it function as normal. In our design, 2kt-E1A is used as a response part to startup viral gene expression and lyse target cells.

We integrate 2kt-E1 with miR885-5p-target and miR663b-target so that while miRNA885-5p and miR663b-target are absent in the cell, E1A can be translated to startup viral gene expression and lyse target cells.

Source

Adenovirus protein

References