Difference between revisions of "Part:BBa K3031002:Design"
| Line 9: | Line 9: | ||
Removal of illegal restriction sites. | Removal of illegal restriction sites. | ||
| − | The 194 amino acids that make up this truncated protein was obtained from the whole protein sequence and was counted backwards from the N-terminal side of the whole cell wall anchor. | + | The 194 amino acids that make up this truncated protein was obtained from the whole protein sequence and was counted backwards from the N-terminal side of the conserved LPxTG motif and low complexity region found on the whole cell wall anchor protein (part BBa_K3031001). |
| Line 17: | Line 17: | ||
The full protein sequence was obtained from the Lp_2578 gene annotation page on NCBI: https://www.ncbi.nlm.nih.gov/gene/?term=lp_2578. | The full protein sequence was obtained from the Lp_2578 gene annotation page on NCBI: https://www.ncbi.nlm.nih.gov/gene/?term=lp_2578. | ||
| − | Information for truncated protein size was obtained from the paper "Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015). | + | Information for truncated protein size was obtained from the paper "Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015). The amino acid sequence for the truncated protein was then derived by counting backwards from the beginning of the conserved LPxTG motif and low complexity region. The result is a protein containing of length 225 amino acids containing 194 amino acids of the Lp_2578 protein + LPxTG anchoring region + low complexity region of 24 amino acids. |
===References=== | ===References=== | ||
Revision as of 05:36, 13 October 2019
CwA (194aa) - Truncated cell wall anchoring protein from Lactobacillus plantarum truncated sequence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removal of illegal restriction sites.
The 194 amino acids that make up this truncated protein was obtained from the whole protein sequence and was counted backwards from the N-terminal side of the conserved LPxTG motif and low complexity region found on the whole cell wall anchor protein (part BBa_K3031001).
Source
The full protein sequence was obtained from the Lp_2578 gene annotation page on NCBI: https://www.ncbi.nlm.nih.gov/gene/?term=lp_2578.
Information for truncated protein size was obtained from the paper "Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells" Moberglisen et al., (2015). The amino acid sequence for the truncated protein was then derived by counting backwards from the beginning of the conserved LPxTG motif and low complexity region. The result is a protein containing of length 225 amino acids containing 194 amino acids of the Lp_2578 protein + LPxTG anchoring region + low complexity region of 24 amino acids.