Difference between revisions of "Part:BBa K143006:Design"
m |
m |
||
Line 5: | Line 5: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The EpsE integration sequences were designed from the EpsE (aka YveO) gene | + | The EpsE integration sequences were designed from the EpsE (aka YveO) gene sequence<cite>#1</cite> and identification of the sequence directly upstream of the gene on the chromosome (found using NCBI's sequence viewer). The upstream and EpsE gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were then produced by PCR cloning with Pfu DNA polymerase |
===Source=== | ===Source=== | ||
Line 14: | Line 14: | ||
<biblio> | <biblio> | ||
− | #1 | + | #1 pmid=9384377 |
</biblio> | </biblio> |
Revision as of 12:45, 18 September 2008
3' Integration sequence for the EpsE locus of B.subtilis
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The EpsE integration sequences were designed from the EpsE (aka YveO) gene sequence#1 and identification of the sequence directly upstream of the gene on the chromosome (found using NCBI's sequence viewer). The upstream and EpsE gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined. The integration sequences were then produced by PCR cloning with Pfu DNA polymerase
Source
The 3’ integration sequence was taken from the B.subtilis chromosome and is homologous to the middle section of the EpsE gene. It was produced by PCR cloning with Pfu DNA polymerase
References
<biblio>
- 1 pmid=9384377
</biblio>