Difference between revisions of "Part:BBa K2989000"

Revision as of 16:43, 17 October 2019

hulc promoter

Long-chain non-coding RNA (long non-coding RNA, lncRNA) High-expression transcripts of liver cancer (highly up-regulated in liver cancer, HULC) are closely related to the occurrence, development, and prognosis of liver cancer. It can also be considered as the diagnostic marker and therapeutic target for early liver cancer.

Usage

We used this Hulc promoter as the upstream control element of our "open" plasmid, and with the promoter-specific ability of the liver cancer cells, the normal cells are more likely to not express the following CeRNA. The expression of the CeRNA is only expressed in the liver cancer tumor cells, and the competitive binding of the miRNA promotes the expression of the HBsAg fragment, thereby eliciting an autoimmune response.

Biology

Highly upregulated in liver cancer (HULC) is a highly expressed lncRNA found in liver cancer tissues. Related studies have shown that HULC is highly expressed in hepatitis B virus (HBV)-associated cirrhosis, which can directly down-regulate the tumor suppressor gene P18, increase the number of regulatory T lymphocytes, and increase the transforming growth factor (transforming growth factor). Factor, TGF) β level, promotes the process of immune response to liver fibrosis. On the other hand, the expression of hulc received transcript levels and down-regulated its activity in interaction with miR-372; the low expression of miR-372 repressed its inhibition of the target protein kinase A catalytic subunit β, induction Phosphorylation of cyclic adenosine CREB; phosphorylated CREB can specifically bind to the CREB binding site in the promoter of HULC gene, maintain the open state of the chromosome structure in the promoter of HULC gene, and increase the transcription of HULC gene and form "Positive feedback adjustment".

Sequence and Features