Difference between revisions of "Part:BBa K3105680:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Sequence was codon optimised for Pichia pastoris
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Sequence was codon optimised for <I>Pichia pastoris</I> <br>
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+
eGFP was added to the C-terminal to develop an easy method to identify AAO-expressing <I>P. patoris</I> clones.
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+
  
 
===Source===
 
===Source===

Revision as of 23:20, 19 October 2019


Expression construct AAO-2A-eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1356
    Illegal BamHI site found at 1543
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2503
    Illegal AgeI site found at 3896
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon optimised for Pichia pastoris
eGFP was added to the C-terminal to develop an easy method to identify AAO-expressing P. patoris clones.

Source

see basic parts

References