Difference between revisions of "Part:BBa K3037001:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Prescission site upstream and downstream.  
+
A PreScission recoginition site was added upstream and downstream of the coding region. This makes it possible to cleave the MBP tag off after purifictation. It can be specifically removed by digest with the PreScission Protease.
  
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Biobrick used in RFC 25 standard
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To make it easy wo use this BioBrick for translational fusion we designed it in the RCF 24 BioBrick standart.
  
 
Codon optimized for <span style="font-style: italic;">E. coli</span> with all forbidden restriction enzyme sites removed
 
Codon optimized for <span style="font-style: italic;">E. coli</span> with all forbidden restriction enzyme sites removed
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===Source===
 
===Source===

Revision as of 23:49, 18 October 2019


Maltose Binding Protein (MBP-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

A PreScission recoginition site was added upstream and downstream of the coding region. This makes it possible to cleave the MBP tag off after purifictation. It can be specifically removed by digest with the PreScission Protease.

To make it easy wo use this BioBrick for translational fusion we designed it in the RCF 24 BioBrick standart.

Codon optimized for E. coli with all forbidden restriction enzyme sites removed

Source

Synthesized by Integrated DNA Technologies (IDT)

References