Difference between revisions of "Part:BBa K3102010:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Latest revision as of 12:14, 21 October 2019
CAE (cellulose acetate esterase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 75
Illegal NgoMIV site found at 1185
Illegal AgeI site found at 205
Illegal AgeI site found at 292
Illegal AgeI site found at 430
Illegal AgeI site found at 613
Illegal AgeI site found at 661
Illegal AgeI site found at 829
Illegal AgeI site found at 1042 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence synthesized and optimized for E.coli on Twist Bioscience
Source
Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1
References
Moriyoshi K, Ohmoto T, Ohe T, Sakai K. "Purification and characterization of an esterase involved in cellulose acetate degradation by neisseria sicca SB". Biosci Biotechnol Biochem. 1999;63(10):1708–13.
Moriyoshi K, Koma D, Yamanaka H, Ohmoto T, Sakai K. "Functional analysis of the carbohydrate-binding module of an esterase from neisseria sicca SB involved in the degradation of cellulose acetate. Biosci Biotechnol" Biochem. 2010;74(9):1940–2.