Difference between revisions of "Part:BBa K3182009"

Revision as of 11:32, 12 October 2019

Parseq-α

This part is a de novo antimicrobial peptide. The antimicrobial peptide was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010.

Figure 1 Shows the antimicrobial activity of Parseq Alpha on E.Coli.. The OD⁶⁰⁰ value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.

Figure 1. Shows the antimicrobial activity of Parseq Alpha on B.Subtilis. The OD⁶⁰⁰ value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 [[File:T--Linkoping_Sweden--AlphaEcoli.png|650px|thumb|center|<b>Figure 1</b> Shows the antimicrobial activity of Parseq Alpha
-  on <i>E.Coli.</i>. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SEM. ]]
+  on <i>E.Coli.</i>. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD. ]]
-[[File:T--Linkoping Sweden--alphaBsubtilis.png|650px|thumb|center|<b>Figure 1.</b> Shows the antimicrobial activity of Parseq Alpha on B.Subtilis. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SEM.]]
+[[File:T--Linkoping Sweden--alphaBsubtilis.png|650px|thumb|center|<b>Figure 1.</b> Shows the antimicrobial activity of Parseq Alpha on B.Subtilis. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.]]
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