Difference between revisions of "Part:BBa K3102021:Design"

(→‎Source)
(→‎Source)
 
Line 14: Line 14:
 
This sequence comes from E.coli and was found through Biocyc:
 
This sequence comes from E.coli and was found through Biocyc:
 
https://biocyc.org/gene?orgid=ECOLI&id=EG11448
 
https://biocyc.org/gene?orgid=ECOLI&id=EG11448
−
 
−
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
 
  
 
===References===
 
===References===

Latest revision as of 11:00, 12 October 2019


ACS (Acetyl-coenzyme A synthetase) Escherichia.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 802
  • 1000
    COMPATIBLE WITH RFC[1000]


=Design Notes

We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.

Source

This sequence comes from E.coli and was found through Biocyc: https://biocyc.org/gene?orgid=ECOLI&id=EG11448

References

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.

Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.