Difference between revisions of "Part:BBa K3102025:Design"
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===Source=== | ===Source=== | ||
Latest revision as of 17:49, 13 October 2019
T7-RBS-TEVp-ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 541
Illegal NgoMIV site found at 847
Illegal AgeI site found at 202 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
TEvp: Sequence synthesized and optimized for E.coli on Geneius
Source
Tobacco etch virus, sequence found through Uniprot https://www.uniprot.org/uniprot/Q0GDU8
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Phan, J., Zdanov, A., Evdokimov, A. G., Tropea, J. E., Peters, H. P. K., Kapust, R. B., Li, M., Wlodawer, A., and Waugh, D. S. (2002). Structural basis for the substrate specificity of tobacco etch virus protease. J. Biol. Chem. 277: 50564-50572.