Difference between revisions of "Part:BBa K3102018:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We mutated an Adenine into Guanine at the | + | We mutated an Adenine into Guanine at the 829bp position (A829G) because of the EcoRI enzyme. |
===Source=== | ===Source=== | ||
Revision as of 09:42, 12 October 2019
MDH (Malate dehydrogenase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 264
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 108
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated an Adenine into Guanine at the 829bp position (A829G) because of the EcoRI enzyme.
Source
This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721
References
Sutherland P. et al., "Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.", J. Bacteriology, 163:1074-1079(1985)v