Difference between revisions of "Part:BBa K3102028:Design"

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===Design Notes===
 
===Design Notes===
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We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme.
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===Source===
 
===Source===

Revision as of 09:35, 12 October 2019


T7-RBS-ACS operon-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 837
    Illegal AgeI site found at 2428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3702


Design Notes

We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme.

Source

None

References

Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55.