Difference between revisions of "Part:BBa K2963009:Design"
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Revision as of 08:08, 12 October 2019
capB*CA - encoding poly-γ-glutamic acid synthetase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1912
Illegal EcoRI site found at 2756
Illegal EcoRI site found at 2815 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1912
Illegal EcoRI site found at 2756
Illegal EcoRI site found at 2815
Illegal NheI site found at 2365
Illegal NheI site found at 2963
Illegal NheI site found at 4281 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1912
Illegal EcoRI site found at 2756
Illegal EcoRI site found at 2815 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1912
Illegal EcoRI site found at 2756
Illegal EcoRI site found at 2815 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1912
Illegal EcoRI site found at 2756
Illegal EcoRI site found at 2815
Illegal NgoMIV site found at 2590 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to express CapBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein, respectively.
Source
Bacillus Genome.
References
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