Difference between revisions of "Part:BBa K2923000"

Latest revision as of 15:52, 20 October 2019

MS2_MS2-PP7_PP7 RNA

Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 coat proteins are able to recognize specifically MS2 and PP7 stem-loops, allowing them to link all parts of the construct. In our project MS2_MS2-PP7_PP7 RNA is used as a positive control for the BH3 system. The sequence between the two MS2 and two PP7 contain 6 differents restrictions sites: PstI, AvaI, XmaI, BsoBI, TspMI, and SmaI. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 25
Illegal PstI site found at 64
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2923000 short</partinfo>
-Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 are able to recognize specifically MS2 and PP7 coat proteins for linked all parts of the construct.
+Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 coat proteins are able to recognize specifically MS2 and PP7 stem-loops, allowing them to link all parts of the construct.
 In our project MS2_MS2-PP7_PP7 RNA is used as a positive control for the BH3 system.
 The sequence between the two MS2 and two PP7 contain 6 differents restrictions sites: PstI, AvaI, XmaI, BsoBI, TspMI, and SmaI.