Difference between revisions of "Part:BBa K2983005"
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This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''. | This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''. | ||
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+ | The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 09:50, 19 October 2019
Ura3 auxotrophic selection marker
This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.
The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 195
Illegal XhoI site found at 228 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 486 - 1000COMPATIBLE WITH RFC[1000]