Difference between revisions of "Part:BBa K2983015"

Revision as of 13:36, 19 October 2019

Loop F-Epsilon

This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology=

The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop F-Epsilon is as fellow : GGTCTCACGCTACAGTGAAGAGC

Figure1: Loop F-Espilon structure

Blue triangle: SapI fixation site (GCTCTTC)

Red triangle : BsaI fixation site (GAGACC)

Blue circle : overhang formed by SapI after cutting (CAG)

Yellow circle: overhang formed by BasI after cutting (CGCT)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal SapI.rc site found at 17

@@ Line 5: / Line 5: @@
 This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
-<!-- Add more about the biology of this part here
+This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
-===Usage and Biology===
+==Usage and Biology===
+The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop F-Epsilon is as fellow : GGTCTCACGCTACAGTGAAGAGC
+[[File:T--Evry Paris-Saclay--Loop F-Epsilon.png|300px|thumb|left| Figure1: Loop F-Espilon structure]]
+Blue triangle: SapI fixation site (GCTCTTC)
+Red triangle : BsaI fixation site (GAGACC)
+Blue circle : overhang formed by SapI after cutting (CAG)
+Yellow circle: overhang formed by BasI  after cutting (CGCT)
 <!-- -->