Difference between revisions of "Part:BBa K3042004"

 
 
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<partinfo>BBa_K3042004 short</partinfo>
 
<partinfo>BBa_K3042004 short</partinfo>
  
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Sequence for a homolog to crystal jelly Aequorea victoria GFP obtained from the pGloTM Plasmid.
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<p>GFP (green fluorescence protein) comes from crystal jelly, Aequorea Victoria, a bioluminescent jellyfish. Aequorea victoria gfp was amplified from a pGloTM Plasmid (Bio-Rad, Hercules, CA, USA). The pGLO plasmid contains gfp and other DNA sequences for gene expression and bacterial transformations.The gfp part contains a SpeI site at 5’-end and a BclI site at 3’-end. This sequence was derived from the University of Connecticut.</p>
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<dl>
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<dt>Primers needed:</dt>
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<dd>gfpNF2- AACTAGTATGGCTAGCAAAGGAGAAGAACTTTTC</dd>
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<dd>gfpNR- TATGATCATCATTTGTAGAGCTCATCCATGC</dd>
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</dl>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:19, 22 October 2019


Dinoflagellate Codon Optimized GFP

GFP (green fluorescence protein) comes from crystal jelly, Aequorea Victoria, a bioluminescent jellyfish. Aequorea victoria gfp was amplified from a pGloTM Plasmid (Bio-Rad, Hercules, CA, USA). The pGLO plasmid contains gfp and other DNA sequences for gene expression and bacterial transformations.The gfp part contains a SpeI site at 5’-end and a BclI site at 3’-end. This sequence was derived from the University of Connecticut.

Primers needed:
gfpNF2- AACTAGTATGGCTAGCAAAGGAGAAGAACTTTTC
gfpNR- TATGATCATCATTTGTAGAGCTCATCCATGC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]