Difference between revisions of "Part:BBa K3282001:Design"

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===References===
 
===References===
 +
 +
1. Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., & Lelie, D. V. D. (2008). Lead(II) resistance in
 +
Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek,
 +
96(2), 171–182. doi: 10.1007/s10482-008-9289-0.
 +
 +
2. Hobman, J. L., Julian, D. J., & Brown, N. L. (2012). Cysteine coordination of Pb(II) is involved in the PbrR-dependent
 +
activation of the lead-resistance promoter, PbrA, from Cupriavidus metallidurans CH34. BMC Microbiology, 12(1), 109. doi:
 +
10.1186/1471-2180-12-109
 +
 +
3. Borremans, B., Hobman, J. L., Provoost, A., Brown, N. L., & van Der Lelie, D. (2001). Cloning and functional analysis of
 +
the pbr lead resistance determinant of Ralstonia metallidurans CH34. Journal of bacteriology, 183(19), 5651–5658.
 +
doi:10.1128/JB.183.19.5651-5658.2001

Latest revision as of 18:42, 11 October 2019


C. metallidurans lead transport protein - pbrT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 643
    Illegal NheI site found at 841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 591
    Illegal NgoMIV site found at 1054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1198
    Illegal SapI.rc site found at 1428


Design Notes

It was codon optimized for E. coli Nissle 1917


Source

NCBI Reference Sequence: NC_006466.1 (115492..117420)

References

1. Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., & Lelie, D. V. D. (2008). Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek, 96(2), 171–182. doi: 10.1007/s10482-008-9289-0.

2. Hobman, J. L., Julian, D. J., & Brown, N. L. (2012). Cysteine coordination of Pb(II) is involved in the PbrR-dependent activation of the lead-resistance promoter, PbrA, from Cupriavidus metallidurans CH34. BMC Microbiology, 12(1), 109. doi: 10.1186/1471-2180-12-109

3. Borremans, B., Hobman, J. L., Provoost, A., Brown, N. L., & van Der Lelie, D. (2001). Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34. Journal of bacteriology, 183(19), 5651–5658. doi:10.1128/JB.183.19.5651-5658.2001