Difference between revisions of "Part:BBa K2983012"
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This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | ||
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+ | ===Usage and Biology=== | ||
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+ | The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop Beta-A is as fellow : GCTCTTCAGCAAGGAGTGAGACC | ||
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+ | [[File:T--Evry Paris-Saclay--Loop B-Alpha.png|300px|thumb|left| Figure1: Loop Beta-A structure]] | ||
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+ | Blue triangle: SapI fixation site () | ||
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+ | Red triangle : BsaI fixation site () | ||
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+ | Blue circle : overhang formed by SapI after cutting () | ||
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+ | red circle: overhang formed by BasI after cutting () | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:24, 19 October 2019
Loop Beta-A
This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop Beta-A is as fellow : GCTCTTCAGCAAGGAGTGAGACC
Blue triangle: SapI fixation site ()
Red triangle : BsaI fixation site ()
Blue circle : overhang formed by SapI after cutting ()
red circle: overhang formed by BasI after cutting ()
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 18
Illegal SapI site found at 1