Difference between revisions of "Part:BBa K2983011"

Revision as of 13:19, 19 October 2019

Loop F-Beta

This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow : GGTCTCACGCTAGCATGAAGAGC

Figure1: Loop F-Beta structure

Blue triangle: SapI fixation site (GAAGAGC)

Red triangle : BsaI fixation site (GGTCTC)

Blue circle : overhang formed by SapI after cutting (GCA)

Yellow circle: overhang formed by BasI after cutting (CGCT)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 9
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal SapI.rc site found at 17

@@ Line 4: / Line 4: @@
 This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
+===Usage and Biology===
+The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow : GGTCTCACGCTAGCATGAAGAGC
+[[File:T--Evry Paris-Saclay--Loop F-Beta.png|300px|thumb|left| Figure1: Loop F-Beta structure]]
+Blue triangle: SapI fixation site (GAAGAGC)
+Red triangle : BsaI fixation site (GGTCTC)
+Blue circle : overhang formed by SapI after cutting (GCA)
+Yellow circle: overhang formed by BasI  after cutting  (CGCT)
 <!-- Add more about the biology of this part here