Difference between revisions of "Part:BBa K2983010"
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This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow :GCTCTTCAATGAGGAGTGAGACC | ||
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| + | [[File:T--Evry Paris-Saclay--Loop Alpha-A.png|200px|thumb|left| Figure 1:Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine)]] | ||
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| + | Blue triangle: SapI fixation site | ||
| + | Red triangle : BsaI fixation site | ||
| + | Blue circle : SapI cutting site | ||
| + | Red circle: BasI cutting site | ||
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| + | <!-- Add more about the biology of this part here | ||
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Revision as of 13:01, 19 October 2019
Loop Alpha-A
This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow :GCTCTTCAATGAGGAGTGAGACC
Blue triangle: SapI fixation site Red triangle : BsaI fixation site Blue circle : SapI cutting site Red circle: BasI cutting site
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 18
Illegal SapI site found at 1