Difference between revisions of "Part:BBa K3282005"
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<partinfo>BBa_K3282005 short</partinfo> | <partinfo>BBa_K3282005 short</partinfo> | ||
− | The part consists of T7 promoter (BBa_I719005), two Anderson RBS (BBa_J61109) separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (BBa_B0012). | + | |
+ | The part consists of T7 promoter [https://parts.igem.org/Part:BBa_I719005 (BBa_I719005)], two Anderson RBS [https://parts.igem.org/Part:BBa_J61109 (Part:BBa_J61109)], separating two coding regions of lead binding protein [https://parts.igem.org/Part:BBa_K3282002 (BBa_K3282002)] and lead transport protein [https://parts.igem.org/Part:BBa_K3282001 (BBa_K3282001)], followed by a TE terminator [https://parts.igem.org/Part:BBa_B0012 (Part:BBa_B0012)]. | ||
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Revision as of 16:54, 17 October 2019
pbrD and pbrT under T7 promoter
The part consists of T7 promoter (BBa_I719005), two Anderson RBS (Part:BBa_J61109), separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (Part:BBa_B0012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1444
Illegal NheI site found at 1642 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1392
Illegal NgoMIV site found at 1855 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1999
Illegal SapI.rc site found at 2229