Difference between revisions of "Part:BBa K3076300:Design"
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===Design Notes===
 
===Design Notes===
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The recombination site was chosen to be near the C terminal of the gene to increase the chance of sucessful knockout.
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The recombination site was chosen to be near the C terminal of the gene to increase the chance of successful knockout.
  
 
===References===
 
===References===

Revision as of 13:12, 13 October 2019


dsDNA substrate with KanR gene for CopA knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The genomic sequence of CopA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence.

Design Notes

The recombination site was chosen to be near the C terminal of the gene to increase the chance of successful knockout.

References