Difference between revisions of "Part:BBa K3076200"
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<p>This part contains the coding sequence of the <i>vanabin2</i> gene which encodes a vanadium-binding protein (VB). The gene was identified from a vanadium-rich sea squirt and it has a function of helping the organism to accumulate metal ion for physiological purpose. The reason for the accumulation is still unsolved.</p> | <p>This part contains the coding sequence of the <i>vanabin2</i> gene which encodes a vanadium-binding protein (VB). The gene was identified from a vanadium-rich sea squirt and it has a function of helping the organism to accumulate metal ion for physiological purpose. The reason for the accumulation is still unsolved.</p> | ||
| − | <p>Although it’s a “vanadium” binding protein, literature reported that copper (II) ions inhibits the binding of vanadium by competing for the binding sites. [1] It showed that VB actually has a higher affinity for copper. Therefore, the VB seems to be an ideal protein for our project to enhance the metal accumulating ability of <i>E. coli</i>.</p> | + | <p>Although it’s a <i>“vanadium” </i>binding protein, literature reported that copper (II) ions inhibits the binding of <i>vanadium</i> by competing for the binding sites. [1] It showed that VB actually has a higher affinity for copper. Therefore, the <i>VB</i> seems to be an ideal protein for our project to enhance the metal accumulating ability of <i>E. coli</i>.</p> |
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Revision as of 17:28, 11 October 2019
Coding sequence of Vanabin2 gene from Ascidia sydneiensis samea
Description
This part contains the coding sequence of the vanabin2 gene which encodes a vanadium-binding protein (VB). The gene was identified from a vanadium-rich sea squirt and it has a function of helping the organism to accumulate metal ion for physiological purpose. The reason for the accumulation is still unsolved.
Although it’s a “vanadium” binding protein, literature reported that copper (II) ions inhibits the binding of vanadium by competing for the binding sites. [1] It showed that VB actually has a higher affinity for copper. Therefore, the VB seems to be an ideal protein for our project to enhance the metal accumulating ability of E. coli.
Usage and Biology
We synthesized the vanabin2 CDS fragment by IDT gBlock and planned to assemble the fragment into pET28a expression vector by Hifi assembly. However, due to time constraints, the work is still in progress.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 90
- 1000COMPATIBLE WITH RFC[1000]