Difference between revisions of "Part:BBa K2952014:Design"
 
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===Design Notes===
 
===Design Notes===
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Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column. Selenocysteine was replaced to cysteine to allow expression in E. coli.
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Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column. Selenocysteine was replaced with cysteine to allow expression in E. coli.
  
  

Latest revision as of 21:29, 10 October 2019


Formate dehydrogenase FDHh from Clostridium carboxidivorans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column. Selenocysteine was replaced with cysteine to allow expression in E. coli.


Source

Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli.

References

Alissandratos, A., et al. (2013). Clostridium carboxidivorans Strain P7T Recombinant Formate Dehydrogenase Catalyzes Reduction of CO2 to Formate. Applied and Environmental Microbiology 79(2) 741-744.

Yoshida A., et al. (2005). Enhanced hydrogen production from formic acid by formate hydrogen lyase‐overexpressing Escherichia coli strains. Appl Environ Microbiol. 2005;71:6762–6768.