Difference between revisions of "Part:BBa K2952014:Design"
(→Source) |
|||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli | + | Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli. |
===References=== | ===References=== | ||
Revision as of 19:55, 10 October 2019
Formate dehydrogenase FDHh from Clostridium carboxidivorans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 451 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column.
Source
Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli.