Difference between revisions of "Part:BBa K3037003"

Revision as of 23:49, 14 October 2019

Fusion protein dCas9 + HRP (MBP/dCas9/linker/HRP/Strep-tag)

Fusion protein
Function	Colour detection of specific DNA sequences
Use in	Escherichia coli
RFC standard	RFC 25 compatible
Backbone	pSB1C3
Submitted by	Team:TU_Dresden 2019[1]

The TU Dresden 2019 team design this biobrick in order to create a system for the short and quick detection of specific DNA sequences.

The plasmid used for the expression of the construct was pSB1C3.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2302
Illegal NheI site found at 5500
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 381
Illegal BglII site found at 5742
Illegal BamHI site found at 4581
Illegal BamHI site found at 5314
Illegal XhoI site found at 5826
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 79

@@ Line 1: / Line 1: @@
-__NOTOC__
 <partinfo>BBa_K3037003 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|Fusion protein
+|-
+|'''Function'''
+|Colour detection of specific DNA sequences
+|-
+|'''Use in'''
+|<span style="font-style: italic;">Escherichia coli</span>
+|-
+|'''RFC standard'''
+|RFC 25 compatible
+|-
+|'''Backbone'''
+|pSB1C3<br>
+|-
+|'''Submitted by'''
+|Team:TU_Dresden 2019[https://2019.igem.org/Team:TU_Dresden]
+|}
+=== Overview ===
 The TU Dresden 2019 team design this biobrick in order to create a system for the short and quick detection of specific DNA sequences.
@@ Line 8: / Line 27: @@
+=== Sequence ===
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3037003 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
-<partinfo>BBa_K3037003 parameters</partinfo>
-<!-- -->