Difference between revisions of "Part:BBa K3038000"
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Bears mainly an ethanol-dehydrogenase activity. | Bears mainly an ethanol-dehydrogenase activity. | ||
| − | === | + | ===Reaction=== |
a secondary alcohol + NAD+ = a ketone + H+ + NADH | a secondary alcohol + NAD+ = a ketone + H+ + NADH | ||
EC:1.1.1.1 | EC:1.1.1.1 | ||
Revision as of 09:45, 10 October 2019
Alcohol dehydrogenase - Geobacillus stearothermophilus cMYC and 6His N-terminal tags
ADR : GenBank: P42327
https://www.ncbi.nlm.nih.gov/protein/P42327
Alcohol dehydrogenase; Short=ADH or ADR
===Thermophilic NAD+-dependent alcohol dehydrogenase===. Bears mainly an ethanol-dehydrogenase activity.
Reaction
a secondary alcohol + NAD+ = a ketone + H+ + NADH EC:1.1.1.1
PROTEIN SEQUENCE
MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK VSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVN KKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFER MEKGKINGRIVLKLKED
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]